Carcinus maenas transcriptome - gill - SRA

SRX713887: Carcinus maenas transcriptome - gill
1 ILLUMINA (Illumina HiSeq 2500) run: 10.1M spots, 2G bases, 1.3Gb downloads

Design: RNA was extracted using Qiagen’s miRNeasy mini kit as per manufacturer’s instructions, with on column DNase digestion. RNA quality and integrity was checked, and quantity measured using Agilent Bioanalyser. cDNA libraries for each tissue were constructed using equal amounts of RNA from four individuals that were pooled together. ERCC Spike-In control mixes were added to control for technical variation during sample preparation and sequencing. mRNA purification was performed via poly(A) enrichment using Tru-Seq low throughput protocol and reagents (Illumina) and finally cDNA libraries constructed using Epicentre’s ScriptSeq v2 RNA-seq library preparation kit.

Submitted by: University of Exeter

Study: Carcinus maenas Transcriptome

PRJNA255867 • SRP046052 • All experiments • All runs

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RNA was isolated from 12 tissues of 4 Carcinus maenas individuals. For every tissue the RNA from each individual was pooled. The RNA was sequenced on an Illumina HiSeq 2500 sequencing machine (Exeter sequencing service: http://ess-wiki.exeter.ac.uk/ess/Exeter_Sequencing_Service). After quality control the RNA was used to produce a de novo assembled transcriptome. The transcriptome was annotated through sequence similarity searches to the NCBI databases. The immune pathways were characterized in detail to provide a basis for further studies.

Sample: GLL01

SAMN03016050 • SRS711835 • All experiments • All runs

Organism: Carcinus maenas

Library:

Name: Cmaenas_gill

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 10.1M spots, 2G bases, 1.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR1589617	10,110,102	2G	1.3Gb	2015-09-29

ID:: 1005215

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